As it is coming close to the time of my presentation. I need to think about what my presentation is going to be composed of. These past couple of weeks, I have learned so much information that I will have no chance of including them into the presentation. So I am deciding to focus on the most important things that I have learned and their purposes. I am going to be talking about the three different types of experiments that I have been doing, In Vitro Transcription(IVT), Cell-Free Translation(CFT), and BCA Assays. IVT and CFT are both procedures that will be easy to explain because they are complimentary of each other. As I have explained the two processes before. CFT is the processes of making proteins by using mRNAs as the blueprint for the construction. However in order to proceed with the CFT, we need to have mRNA to start with. This means that we would need to synthesize it. That is where IVT comes into play, IVT is the process of taking a linearized plasmid that contains the DNA sequence that we want to make the complimentary mRNA of and making a transcription reaction. The transcription reaction will then result in mRNA that is complementary to our plasmid. However, the result of the reaction is not just pure mRNA. In the sample we will also have the plasmids that we used as the template for transcription. So we then have to treat the sample to enzymes that break down the DNA and the clean the sample to get rid of the DNA and end up with mRNA with exceptional purity. After the treatment and purification is done, we proceed to use that mRNA in CFT. The CFT reaction isn't like the IVT reaction in which we need to treat it with DNAse and purify it. We only need to prep the reaction and incubate it at 37C( Body Temp.) to initiate translation. After the incubation is done and the reaction is complete, we freeze the sample until analytics will be ran on them. I personally never have observed or done this step before. The biologist that will analyize the sample will set up a western blot. I western blot is a gel electropheresis experiment in which which we place the samples in the wells of the gel and have it travel through the gel. The equipment that is used for this experiment creates a electrical field that the gel sits in and at one side the gel is positive and at the other it is negative. You would place the samples on the negatively charged portion of the field and have it travel through the gel towards the positive side. What I just explained right now is going to be the general pieces of information that I will present in my presentation. I am still figuring out how I will incorporate BCA assays into my presentation however.