Today I had my first teacher visit at Arcturus. I had communicated, planned, and reserved time before hand with my mentor to have this meeting. The meeting went as expected. We discussed the types of work that I have done the past week and what I can look forward to doing in the future. My mentor stressed that the biggest contribution that I will make to the company is performing In Vitro Transcription. He stated that eventually, I will be independently be having tasks to complete after my training.
In Vitro Transcription is a procedure that results in mRNA as it's final product. "In Vitro" means within a test tube, meaning that we will transcribe mRNA within a test tube. The process of In Vitro Transcription starts with a template of DNA that we will eventually then mix with our other contents. Polymerase also will be in the mix. The polymerase are what transcribes and pieces together the mRNA. They attach to the promoter site on the DNA template then travels along the template creating the mRNA simultaneously.
Some other points that my mentor mentioned in our meeting was the analysis of an experiment. He stressed that a experiment will not be considered complete until the analysis and write up is complete for it. Luckily, I have already practiced creating a analysis for a experiment I've done earlier this week, I will be able to use that analysis as a reference for my future write-ups that I will make. He also mentioned that I will work with In Vitro Translation. In Vitro Translation, just like In Vitro Transcription, will be performed within a sample tube however instead of producing the mRNAs, we will mix them with lysate to result in the production of proteins from our mRNA.
My overall thoughts on the meeting was that it went well. Both my teacher and mentor seemed like they were on the same page of what is expected of this internship and of me. I am excited to see what the future has in store for me.
In Vitro Transcription is a procedure that results in mRNA as it's final product. "In Vitro" means within a test tube, meaning that we will transcribe mRNA within a test tube. The process of In Vitro Transcription starts with a template of DNA that we will eventually then mix with our other contents. Polymerase also will be in the mix. The polymerase are what transcribes and pieces together the mRNA. They attach to the promoter site on the DNA template then travels along the template creating the mRNA simultaneously.
Some other points that my mentor mentioned in our meeting was the analysis of an experiment. He stressed that a experiment will not be considered complete until the analysis and write up is complete for it. Luckily, I have already practiced creating a analysis for a experiment I've done earlier this week, I will be able to use that analysis as a reference for my future write-ups that I will make. He also mentioned that I will work with In Vitro Translation. In Vitro Translation, just like In Vitro Transcription, will be performed within a sample tube however instead of producing the mRNAs, we will mix them with lysate to result in the production of proteins from our mRNA.
My overall thoughts on the meeting was that it went well. Both my teacher and mentor seemed like they were on the same page of what is expected of this internship and of me. I am excited to see what the future has in store for me.